AustralieFrV1, Main, Exploration, bibRecord, 007062

Specific cellular water dynamics observed in vivo by neutron scattering and NMR

Identifieur interne : 007062 ( Main/Exploration ); précédent : 007061; suivant : 007063

Specific cellular water dynamics observed in vivo by neutron scattering and NMR

Auteurs : Marion Jasninthe Authors Contributed Equally To The Work. [Allemagne] ; Andreas Stadler [Allemagne] ; Moeava Tehei [Australie] ; Giuseppe Zaccai [France]

Source :

Physical Chemistry Chemical Physics [ 1463-9076 ] ; 2010.

RBID : ISTEX:D16D2433678642AE86CFB547014CDB4B7C86E440

English descriptors

Abstract

Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (ngstrm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not tamed by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration water an intracellular water component was observed with significantly slowed down translational diffusion. The results are discussed and compared to observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMRa method that is sensitive to water rotational dynamics on a wide range of time scales.

Url:

https://api.istex.fr/document/D16D2433678642AE86CFB547014CDB4B7C86E440/fulltext/pdf

DOI: 10.1039/c0cp01048k

Affiliations:

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Le document en format XML

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<author wicri:is="90%"><name sortKey="Stadler, Andreas" sort="Stadler, Andreas" uniqKey="Stadler A" first="Andreas" last="Stadler">Andreas Stadler</name>
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<term>Blood cells</term>
<term>Bulk water</term>
<term>Cell water</term>
<term>Cellular components</term>
<term>Chem</term>
<term>Coli</term>
<term>Coli samples</term>
<term>Deuterium labelling</term>
<term>Dynamics</term>
<term>Energy resolution</term>
<term>Exponential function</term>
<term>Extreme halophile archaeon</term>
<term>Extreme halophiles</term>
<term>Extreme halophilic archaea</term>
<term>Force constants</term>
<term>Haloarcula marismortui</term>
<term>Halophile</term>
<term>Halophilic</term>
<term>Hydrated</term>
<term>Hydrated powder</term>
<term>Hydrated powders</term>
<term>Hydration</term>
<term>Hydration shells</term>
<term>Hydration water</term>
<term>Immobile fraction</term>
<term>Incoherent neutron</term>
<term>Internal motions</term>
<term>Intracellular</term>
<term>Intracellular environment</term>
<term>Intracellular water</term>
<term>Intracellular water dynamics</term>
<term>Iris</term>
<term>Line results</term>
<term>Lled</term>
<term>Membrane surfaces</term>
<term>Molecular biophysics</term>
<term>Natural abundance</term>
<term>Neutron</term>
<term>Neutron spectrometers</term>
<term>Normal temperature behaviour</term>
<term>Other atoms</term>
<term>Owner societies</term>
<term>Phys</term>
<term>Protein dynamics</term>
<term>Pure water</term>
<term>Qens</term>
<term>Qens analysis</term>
<term>Qens experiments</term>
<term>Relaxation rate</term>
<term>Residence time</term>
<term>Residence times</term>
<term>Rotational</term>
<term>Rotational correlation times</term>
<term>Similar conclusion</term>
<term>Single atoms</term>
<term>Slow water component</term>
<term>Spectrometer</term>
<term>Tehei</term>
<term>Temperature dependence</term>
<term>Time scale</term>
<term>Time scales</term>
<term>Time window</term>
<term>Translational</term>
<term>Translational motions</term>
<term>Uctuation amplitudes</term>
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<term>Water dynamics</term>
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<term>Biological function</term>
<term>Biophys</term>
<term>Blood cells</term>
<term>Bulk water</term>
<term>Cell water</term>
<term>Cellular components</term>
<term>Chem</term>
<term>Coli</term>
<term>Coli samples</term>
<term>Deuterium labelling</term>
<term>Dynamics</term>
<term>Energy resolution</term>
<term>Exponential function</term>
<term>Extreme halophile archaeon</term>
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<term>Hydrated powder</term>
<term>Hydrated powders</term>
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<term>Hydration shells</term>
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<term>Intracellular water dynamics</term>
<term>Iris</term>
<term>Line results</term>
<term>Lled</term>
<term>Membrane surfaces</term>
<term>Molecular biophysics</term>
<term>Natural abundance</term>
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<term>Owner societies</term>
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<term>Protein dynamics</term>
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<term>Qens analysis</term>
<term>Qens experiments</term>
<term>Relaxation rate</term>
<term>Residence time</term>
<term>Residence times</term>
<term>Rotational</term>
<term>Rotational correlation times</term>
<term>Similar conclusion</term>
<term>Single atoms</term>
<term>Slow water component</term>
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<term>Temperature dependence</term>
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<term>Uctuation amplitudes</term>
<term>Water activity</term>
<term>Water dynamics</term>
<term>Water molecule</term>
<term>Water molecules</term>
<term>Water motions</term>
<term>Water protons</term>
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<front><div type="abstract">Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (ngstrm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not tamed by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration water an intracellular water component was observed with significantly slowed down translational diffusion. The results are discussed and compared to observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMRa method that is sensitive to water rotational dynamics on a wide range of time scales.</div>
</front>
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Specific cellular water dynamics observed in vivo by neutron scattering and NMR

Specific cellular water dynamics observed in vivo by neutron scattering and NMR

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English descriptors

Abstract

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